Study of AcOH - HCOOH - H2O - CHCl3 solvent system application to the separation of two large and hydrophobic fragments of bacteriorhodopsin membrane protein by centrifugal partition chromatography
Summary
Type: HPLC Application #: 108160
Detection: UV, 216nm Conditions: eluent: gradient from 40 to 100% ethanol in 0.1M H3PO4 in water within 20min flow rate 0.7ml/min Authors: Foucault, A. et al. Source: J. Liquid Chromatogr. 11 (1988) 2455-2472
Details
Legend: Fig. 6. HPLC chromatogram of upper and lower phases for point c (AcOH / HCOOH /
H2O / CHCl3 33.3:5.6:16.7:44.4), retention times: C2 16min, C1 20min.